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KMID : 0616619990050020273
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Journal of Soonchunhyang Medical College
1999 Volume.5 No. 2 p.273 ~ p.280
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Immunohistochemical study for transforming growth factor βs in the retinal pigment epithelium following laser photocoagulation in the rat
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Abstract
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Among the various growth factors produced by the retinal pigment epithelium (RPE) after laser photocoagulation, transforming growth factor (TGF) β is known as a strong inhibitor of vascular endothelial cell proliferation. Immunohistochemical staining of the photocoagulated retina was done to evaluate the effects of laser photocoagulation on TGF β production and the relative intensity of immunoreactivity between different TGF β subtypes (β1, β2, β3).
Argon blue-green laser photocoagulation was performed on the eyes of 20 Sprague-Dawley rate (40 eyes) and 5 rats (10 eyes) were used as the untreated group. The eyes were enucleated on day 3, 7, 14, 28 after laser photocoagulation and enrolled into the analysis of immunohistochemical localization of TGF βs.
Immunoreactivity for TGF β1 & β2 in photocoagulated RPE was intense on day 3 and the intensity increased on day 7 after laser photocoagulation. However, the intensity decreased on day 14, but immunoreactivity still was present until day 28. The immunoreactivity was more intense for TGF β2 than β1 and difference in the intensity became more prominent after 14 days. No immunoreactivity was identified for TGF β3 in photocoagulated retina throughout 28 days.
Laser photocoagulation increases production of TGF β1 & β2 at the laser burn site and surrounding RPE. Immunoreactivity to TGF β2 was more intense than that of TGF β1 and also lasted longer. This finding demonstrates that TGF β2 participates in the healing process of photocoagulated RPE and it seems that TGF β2, which inhibits endothelial cell proliferation, plays the most important role in regression of neovascularization.
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